小鼠 anti-大鼠 Ig (Chain lambda), (Light Chain) Antibody
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- 抗原 See all Ig products
- Ig
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抗原表位
- Chain lambda, Light Chain
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 纯化方法
- Affinity Chromatography
- 免疫原
- Rat lambda light chain. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the rat PAI-O myeloma cell line.
- 克隆位点
- MARL-15
- 亚型
- IgG1
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Goat anti-Rat Ig (Heavy & Light Chain) antibody
ELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Rat Ig (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat Ig (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Rat Ig (Heavy & Light Chain) antibody (Biotin)ELISA, FACS, IHC, WB Polyclonal IgG Biotin
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- 应用备注
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Immunohistochemistry on frozen sections. ELISA. Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Ig
- 别名
- Immunoglobulin (Ig 产品)
- 背景
- All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5 kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L, Lambda, 0.4-4.2 mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
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