This antibody recognises CD49F (VLA-6 alpha chain). Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin.
纯化方法
Affinity chromatography on Protein G
免疫原
BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
Flow cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.1 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD49f is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6