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Avidin 抗体 (HRP)

Details for Product anti-Avidin Antibody No. ABIN116963, 供应商: Log in to see
抗原
  • avidin
  • avd
  • LOC723980
  • AVD
适用
小鸡
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26
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宿主

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2
克隆类型
多克隆
标记
This Avidin antibody is conjugated to HRP
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10
9
4
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1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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10
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2
2
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1
Supplier
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免疫原 Avidin [Hen Egg White].
特异性 This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White ]. Cross reactivity against Avidin from other sources is unknown.
纯化方法 Immunoaffinity chromatography.
背景 The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
基因ID 396260
NCBI登录号 NP_990651
UniProt P02701
研究领域 Epitope Tags, Tags/Labels
应用备注 Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: This product has been assayed against 1.0 μg of Avidin [Hen EggWhite ] in a standard capture ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,000 to 1: 27,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 2.0 mL of deionized water (or equivalent).
浓度 10.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative.
储存液 Gentamicin sulfate
注意事项 This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件 4 °C/-20 °C
储存方法 Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
背景 Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).