Myc 标记 抗体

ABIN116942 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主

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克隆类型
多克隆
标记
非结合性
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应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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选项
Supplier
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免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. Remarks: The sequence corresponds to aa 410-419 of Human c-Myc.
序列 EQKLISEEDL
特异性 Anti-Myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
纯化方法 Affinity Chromatography.
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. We offer anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Please visit our website at www.acris-antibodies.de for more informations.Synonyms: c-myc tag, myc tag, myc-tag
基因ID 4609
NCBI登录号 NP_002458
UniProt P01106
研究领域 Transcription Factors, Cancer, Cell Cycle
应用备注 This antibody has been tested by ELISA (1/135,000) and Western blot (1/500-1/5,000)against both the immunizing peptide and myc containing recombinant proteins. Use1/400-1/2,000 for Immunohistochemistry on frozen sections. Although not tested, this antibody is likely functional for Immunoprecipitation andImmunocytochemistry.
限制 仅限研究用
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative without stabilizers.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 -20 °C
储存方法 Store the antibody (in aliquots) at -20 °C.
厂商提供的图像
Western Blotting (WB) image for anti-Myc Tag antibody (ABIN116942) Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal link...
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