beta-2 Microglobulin 抗体 (HRP)
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- 抗原 See all beta-2 Microglobulin (B2M) 抗体
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This beta-2 Microglobulin antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM)
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi- step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified beta-2-Microglobulin [Human Urine]. Cross reactivity against beta-2-Microglobulin from other sources is unknown.
- 纯化方法
- Multistep Process.
- 免疫原
- Beta-2-Microglobulin [Human Urine].
- Top Product
- Discover our top product B2M Primary Antibody
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- 应用备注
- Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer
- 储存液
- Gentamicin sulfate
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below.
- 有效期
- 12 months
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- 抗原
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
- 别名
- Beta-2-microglobulin (B2M 产品)
- 别名
- Ly-m11 antibody, beta2-m antibody, beta2m antibody, bwm antibody, wu:fa94c05 antibody, wu:fb10a09 antibody, b2m-W01 antibody, b2m-W03 antibody, b2m-Z01 antibody, b2m-Z02 antibody, b2m-Z03 antibody, B2M antibody, b2m antibody, B2MG antibody, wu:fb66f11 antibody, wu:fj01f08 antibody, zgc:64161 antibody, b-2-m antibody, beta-2-microglobulin antibody, beta-2 microglobulin antibody, beta-2-microglobulin S homeolog antibody, beta-2-microglobulin, like antibody, B2M antibody, B2m antibody, b2m antibody, b2m.S antibody, b2ml antibody
- 背景
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Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Alternate names: B2M, Beta 2 microglobulin, Beta-2 microglobulin - 基因ID
- 567
- NCBI登录号
- NP_004039
- UniProt
- P61769
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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