EpCAM 抗体 (FITC)
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Quick Overview for EpCAM 抗体 (FITC) (ABIN111179)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen or EPG34). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).Polypeptide reacting:
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- HT-29 colon carcinoma cell line
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亚型
- IgG1
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibodyEPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibodyEPCAM 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) antibodyEPCAM 适用: 人, 小鼠 WB, FACS, IHC, IF 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 289-314), (Cytoplasmic Domain) antibodyEPCAM 适用: 人, Cat, 犬 IHC 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, IP, ICC 宿主: 兔 Monoclonal BLR192J unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 264-314) antibodyEPCAM 适用: 人, 小鼠 WB, FACS, IHC, IP, ICC, IF (p) 宿主: 兔 Monoclonal BLR077G unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 264-314) antibodyEPCAM 适用: 人, 小鼠 WB, FACS, IHC, IP, ICC, IF (p) 宿主: 兔 Monoclonal BLR077G unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibodyEPCAM 适用: 人, Cat, 犬, 豚鼠 WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated
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应用备注
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Immunofluorescence Microscopy. Cell Sorting, Cytological Material. Immunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. With Paraffin embedded sections, protease pretreatment is required prior to antibodyapplication. Working Dilution: Dilute at least 1/10 with PBS, pH 7.4 for Immunohistochemicalapplication. Incubation Time: 1 h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 3.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- CD326 / EPCAM / TACSTD1
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背景
- Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
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基因ID
- 4072
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NCBI登录号
- NP_002345
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UniProt
- P16422
抗原
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