Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116).
Immunoblotting. Cell sorting. Cytological material. Immunofluorescence microscopy. Immunohistochemistry on frozen and paraffin-embedded tissue. With paraffin embeddedsections, protease pre-treatment is required prior to antibody application. Dilute at least 1: 10 with PBS, pH 7.4, for immunohistochemical application. Incubationtime: 1 h at RT, extended with paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.