V5 Epitope 标记 (AA 95-108) 抗体

ABIN1104955 产品详细信息, 供应商: Log in to see
抗原
抗原表位
AA 95-108
14
适用
标记
403
18
16
8
3
3
宿主

249
143
37
7
5
4
3
1
1
克隆类型
多克隆
标记
非结合性
31
28
21
14
13
12
12
12
11
10
10
10
10
9
8
8
7
6
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
369
263
137
95
78
71
52
44
28
13
9
8
5
4
4
2
2
2
2
1
1
1
选项
Supplier
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免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.

Type of Immunogen: Synthetic peptide
序列 GKPIPNPLLG LDST
亚型 IgG
特异性 This affinity-purified antibody is directed against V5 motif and is useful in determining its presence in various assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.
纯化方法 Affinity purified
过滤 Sterile filtered
别名 V5 Tag
物质类 Tag
研究领域 Tags/Labels
应用备注 Approved: ELISA, IHC (1:500 - 1:3000), WB (1:5000 - 1:10000)

Usage: Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Aliquot to Avoid freeze/thaw cycles. Store undiluted.
储存条件 4 °C,-20 °C
储存方法 Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
厂商提供的图像
 image for anti-V5 Epitope Tag (AA 95-108) antibody (ABIN1104955) anti-V5 Epitope Tag (AA 95-108) antibody
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