The monoclonal antibody MN-8C4 binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the a and b monomers. The antibody also cross-reacts with Zrp from rainbow trout (Oncorhynchus mykiss), Arctic charr (Salvelinus alpinus), turbot (Scophthalmus maximus) and cod (Gadus morhua). The degree of cross-reactivity differs between species and with the methods employed.
免疫原
Immunogen source:Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).
The monoclonal antibody MN-8C4 works well in both ELISA and western blot. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: ELISA: 1:100 - 1: 5000. Western blot: 1:100 - 1: 1000
限制
仅限研究用
状态
Liquid
储存条件
4 °C
抗原
Zona Radiata Protein
别名
zrp antibody, zona radiata protein antibody, zrp antibody
背景
This package contains one vial of 100 µl antibody solution. The antibody is dissolved in 0.1 M Na-phosphate buffer, 0.15 M NaCl, 1 % BSA, 12.3 mM Na-azide, pH 7.4.