Anti-DYKDDDDK 标记 (L5) Affinity Gel

ABIN2669061 产品详细信息, 供应商: Log in to see
蛋白/底物
适用
标记
宿主
大鼠
克隆类型
单克隆
应用范围
Multiplex Assay (MA)
选项
Supplier
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产品特性 The anti-DYKDDDDK tag affinity gel is composed of the L5 antibody conjugated to an agarose resin, which can be used for purification of proteins expressing the octapeptide, DYKDDDDK. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The binding capacity of the L5 agarose resin is greater than 0.8 mg/mL. The resin can be re-used at least five times (see technical bulletin for regeneration and storage). This affinity gel is also useful for immunoprecipitation of DYKDDDDK-tagged protein.
纯化方法 The antibody was purified by affinity chromatography.
组件 Anti-DYKDDDDK Tag (L5) Affinity Gel
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Liquid
缓冲液 50 % anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09 % Sodium azide. The volume specified for each catalog number indicates the volume of resin included.
储存条件 4 °C
储存方法 Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
有效期 12 months
厂商提供的图像
ELISA image for Anti-DYKDDDDK Tag (L5) Affinity Gel (ABIN2669061) Anti-DYKDDDDK Tag (L5) Affinity Gel
Western Blotting (WB) image for Anti-DYKDDDDK Tag (L5) Affinity Gel (ABIN2669061) Anti-DYKDDDDK Tag (L5) Affinity Gel (Image 2)
背景 Grimley, Polyakova, Vamathevan, McKenary, Hayes, Patel, Smith, Bridges, Fosberry, Bhardwaja, Mouzon, Chung, Barrett, Richmond, Modha, Solari: "Over expression of wild type or a catalytically dead mutant of Sirtuin 6 does not influence NF?B responses." in: PLoS ONE, Vol. 7, Issue 7, pp. e39847, 2012 (PubMed).