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WDR17 Protein (AA 1-1322) (Strep Tag)

This Recombinant WDR17 protein is expressed in Cell-free protein synthesis (CFPS).
产品编号 ABIN3096334
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for WDR17 Protein (AA 1-1322) (Strep Tag) (ABIN3096334)

抗原

WDR17 (WD Repeat Domain 17 (WDR17))

蛋白类型

Recombinant

宿主

资源

Cell-free protein synthesis (CFPS)

应用范围

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

纯度

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

质量等级

custom-made
  • 产品特性

    AA 1-1322

    标记

    This WDR17 protein is labelled with Strep Tag.

    序列

    MAWMTYISNW FEQDDWYEGL QRANMSQVRQ VGLLAAGCQP WNKDVCAASG DRFAYCATLA IYIYQLDHRY NEFKLHAIMS EHKKTITAIS WCPHNPDLFA SGSTDNLVII WNVAEQKVIA KLDSTKGIPA SLSWCWNAED VVAFVSHRGP LFIWTISGPD SGVIVHKDAH SFLSDICMFR WHTHQKGKVV FGHIDGSLSI FHPGNKNQKH VLRPESLEGT DEEDPVTALE WDPLSTDYLL VVNLHYGIRL VDSESLSCIT TFNLPSAAAS VQCLAWVPSA PGMFITGDSQ VGVLRIWNVS RTTPIDNLKL KKTGFHCLHV LNSPPRKKFS VQSPTKNHYT SSTSEAVPPP TLTQNQAFSL PPGHAVCCFL DGGVGLYDMG AKKWDFLRDL GHVETIFDCK FKPDDPNLLA TASFDGTIKV WDINTLTAVY TSPGNEGVIY SLSWAPGGLN CIAGGTSRNG AFIWNVQKGK IIQRFNEHGT NGIFCIAWSH KDSKRIATCS SDGFCIIRTI DGKVLHKYKH PAAVFGCDWS QNNKDMIATG CEDTNVRVYY VATSSDQPLK VFSGHTAKVF HVKWSPLREG ILCSGSDDGT VRIWDYTQDA CINILNGHTA PVRGLMWNTE IPYLLISGSW DYTIKVWDTR EGTCVDTVYD HGADVYGLTC HPSRPFTMAS CSRDSTVRLW SLTALVTPVQ INILADRSWE EIIGNTDYAI EPGTPPLLCG KVSRDIRQEI EKLTANSQVK KLRWFSECLS PPGGSDNLWN LVAVIKGQDD SLLPQNYCKG IMHLKHLIKF RTSEAQELTT VKMSKFGGGI GVPAKEERLK EAAEIHLRLG QIQRYCELMV ELGEWDKALS IAPGVSVKYW KKLMQRRADQ LIQEDKDDVI PYCIAIGDVK KLVHFFMSRG QLKEALLVAQ AACEGNMQPL HVSVPKGASY SDDIYKEDFN ELLHKVSKEL AEWYFQDGRA VLAACCHLAI DNIELAMAYL IRGNELELAV CVGTVLGESA APATHYALEL LARKCMMISV CFPCVGYSVP FCYVNRNLAA DLLLMIPDNE LHLIKLCAFY PGCTEEINDL HDKCKLPTVE ECMQLAETAR ADDNIFETVK YYLLSQEPEK ALPIGISFVK EYISSSDWTL DTIYPVLDLL SYIRTEKLLL HTCTEARNEL LILCGYIGAL LAIRRQYQSI VPALYEYTSQ LLKRREVSVP LKIEYLSEEL DAWRACTQST NRSLEDSPYT PPSDSQRMIY ATLLKRLKEE SLKGIIGPDY VTGSNLPSHS DIHISCLTGL KIQGPVFFLE DGKSAISLND ALMWAKVNPF SPLGTGIRLN PF
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    产品特性

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • 应用备注

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -80 °C

    储存方法

    Store at -80°C.

    有效期

    12 months
  • 抗原

    WDR17 (WD Repeat Domain 17 (WDR17))

    别名

    WDR17

    背景

    WD repeat-containing protein 17

    分子量

    147.7 kDa

    UniProt

    Q8IZU2
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