Human Normal Tissue Blot VI
WB
宿主: Human
产品编号 ABIN2455967
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Human Normal Tissue Blot VI (ABIN2455967)
宿主
Human
应用范围
Western Blotting (WB)
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产品特性
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: left cerebellum, 3: right cerebellum, 4: frontal lobe, 5: occipital lobe, 6: parietal lobe, 7: whole eye, 8: spinal cord, 9: temporal lobe, and 10: thalamus.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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应用备注
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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限制
- 仅限研究用
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注意事项
- The blot can be reused after stripping.
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储存条件
- 4 °C
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储存方法
- Store at 4 °C.
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