Human Normal Tissue Blot V
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北京 101111
Quick Overview for Human Normal Tissue Blot V (ABIN2455966)
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应用范围
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产品特性
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are appendix, kidney, liver, lymph node, peripheral blood leukocyte, spleen, thymus, tonsil, and thyroid.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: appendix, 3: kidney, 4: liver, 5: lymph node, 6: peripheral blood leukocyte, 7: spleen, 8: thymus, 9: tonsil, and 10: thyroid
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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应用备注
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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限制
- 仅限研究用
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注意事项
- The blot can be reused after stripping.
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储存条件
- 4 °C
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储存方法
- Store at 4 °C.
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