Quick Overview for Human Normal Tissue Blot I (ABIN2455962)
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应用范围
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产品特性
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.
Sample diagnosis: Normal
Samples loaded: Lanes: 1: protein markers, 2: brain, 3: colon, 4: heart, 5: kidney, 6: liver, 7: lung, 8: pancreas, and 9: spleen.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Rat Tail
宿主: 大鼠
Guinea Pig Spinal Cord宿主: 豚鼠 Normal Spinal Cord
Sheep Red Blood Cells (10% Washed Pooled Cells)H 宿主: 绵羊 Normal Red Blood Cells
Mouse BrainIEM, IHC, WB 宿主: 小鼠 Normal Brain
Chicken Red Blood Cells (10% Washed Pooled Cells)H 宿主: 小鸡 Normal Red Blood Cells
Hamster Complement (Lyophilized) With Diluent宿主: 仓鼠 Normal Complement
Guinea Pig Complement (Lyophilized) With Diluent宿主: 豚鼠 Normal Complement
Rabbit Blood (In Sodium Citrate)H 宿主: 兔 Normal Blood
Sheep Red Blood Cells (100% Washed Pooled Cells)H 宿主: 绵羊 Normal Red Blood Cells
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应用备注
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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限制
- 仅限研究用
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注意事项
- The blot can be reused after stripping.
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储存条件
- 4 °C
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储存方法
- Store at 4 °C.
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