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TDC2 抗体 (C-Term)

This anti-TDC2 antibody (ABIN4889606) is a Rabbit Polyclonal antibody detecting TDC2 in ELISA, WB, IHC. Suitable for Drosophila melanogaster.
产品编号 ABIN4889606
发货至: 中国

Quick Overview for TDC2 抗体 (C-Term) (ABIN4889606)

抗原

TDC2 (Tyrosine Decarboxylase 2 (TDC2))

适用

Drosophila melanogaster

宿主

克隆类型

多克隆

应用范围

ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

    C-Term

    特异性

    Reacts with Drosophila melanogaster 72 kDa Tdc2 protein

    纯化方法

    Purified (protein A)

    免疫原

    Synthetic peptide derived from C-terminal part of Drosophila Tdc2 protein.
  • 应用备注

    Working dilution: Optimal dilutions should be determined by the end user.
    The following are guidelines only:
    IHC(1:200 - 1:1000) WB(1:200 - 1:2000)

    限制

    仅限研究用
  • 生效 #100812 (Immunofluorescence)
    '独立验证'标志
    by
    Department of Entomology, University of California, Riverside
    No.
    #100812
    日期
    2017.05.26
    抗原
    Tdc2
    Lot Number
    13B1
    Method validated
    Immunofluorescence
    Positive Control

    D. melanogaster octopaminergic neurons labeled with Tdc2-Gal4 of the abdominal nerve to the ovary

    Negative Control
    Notes

    Passed. ABIN809182 labels octopaminergic neurons specifically and with no background.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN4889606
    Secondary Antibody
    goat anti-rabbit AF542 conjugated antibody (Life Technologies)
    Full Protocol
    • Dissect ovaries of D. melanogaster ETHR-Gal4/UAS-MCD8-GFP expressing GFP in octopaminergic neurons in cold Schneider’s Insect Medium (S2; Sigma Aldrich, S01416).
    • Transfer tissue to 2ml protein LoBind tubes (Eppendorf, 022431102) filled with S2 containing 2% paraformaldehyde (PFA) at RT.
    • Fix tissue for 55min at RT while nutating.
    • Wash tissue 4x 10min with 1.75ml PBS containing 0.5% Triton X-100 (PBST).
    • Remove PBST and add 200µl 5% goat serum (GS; Thermo Fisher Scientific, 16210064) in PBST per tube.
    • Incubate 1.5h at RT on a rotator.
    • Remove blocking solution.
    • Incubate with primary
      • rabbit anti-Tdc2 antibody (Tyrosine Decarboxylase 2) (C-Term) (antibodies-online, ABIN4889606, lot 13B1) diluted 1:200 in blocking solution.
      • mouse anti-GFP (Thermo Fisher Scientific) diluted 1:500 in blocking solution.
    • Incubate for 4h at RT followed by 36-48h at 4°C on a rotator.
    • Rinse tissue with 1.75ml PBST. Allow the tissue to settle to the bottom before removing the liquid.
    • Wash tissue 3x 30min with 1.75ml PBST.
    • Incubate with secondary 200µl secondary goat anti-rabbit AF542 conjugated antibody (Life Technologies) and goat anti-mouse AF488 conjugated antibody (Life Technologies, A11034) diluted 1:500 in blocking solution containing 0.5mg/ml DAPI.
    • Incubate for 4h at RT followed by 72h at 4°C on a rotator.
    • Rinse tissue with 1.75ml PBST. Allow the tissue to settle to the bottom before removing the liquid.
    • Wash tissue 3x 30min with 1.75ml PBST.
    • Add 1.75ml PBST containing 4% PFA at RT.
    • Fix tissue for 5h at RT while nutating.
    • Rinse tissue with 1.75ml PBST. Allow the tissue to settle to the bottom before removing the liquid.
    • Wash tissue 4x 15min with 1.75ml PBST.
    • Mount tissue on a poly-L-lysine (Sigma Aldrich, P1524-25MG) coated cover glass.
    • Dehydrate tissue by covering the cover glass for 10min each with 30%, 50%, 75%, 95%, 100%, 100%, and 100% EtOH.
    • Clearing using by covering the cover glass 3x 5min with xylene.
    • Add 7 drops of dibutyl phthalate in xylene (DPX) on top of the mounted tissue.
    • Seat cover glass face down gently onto a prepared slide with spacers.
    • Let the slide dry for 48h at RT in a hood before viewing.
    Experimental Notes
    • Staining of ETHR-Gal4/UAS-MCD8-GFP expressing GFP in octopaminergic neurons was performed as previously described.

    • ABIN4889606 worked fantastically. It labeled octopaminergic neurons specifically and with no background. Pictures are below. The well-characterized neurons labeled with Tdc2-Gal4 of the abdominal nerve to the ovary described in Middleton et al. (2006) overlapped with ABIN4889606. Staining with ABIN4889606 was stronger than Gal4 labeling.

  • 状态

    Lyophilized

    溶解方式

    Must be reconstituted in distilled water.

    浓度

    1 mg/mL

    缓冲液

    Tris 0,1M, glycine 0,1M, sucrose 2 %

    储存条件

    4 °C/-20 °C

    储存方法

    Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

    有效期

    24 months
  • 抗原

    TDC2 (Tyrosine Decarboxylase 2 (TDC2))

    别名

    Tyrosine Decarboxylase 2

    背景

    Enzyme involved in tyrosine metabolism. Use Pyridoxal phosphate as cofactor.

    基因ID

    246620

    UniProt

    A1Z6N4
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