Spectrin, Alpha, beta 抗体
-
- 抗原
- Spectrin, Alpha, beta
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity with Spectrin from other sources has not been determined.
- 纯化方法
- Anti-Spectrin antibody was purified from mouse ascites by protein A chromatography. This product reacts with human.
- 免疫原
-
Immunogen: Spectrin(alpha and beta) antibody was produced in mice by repeated immunizations with human erythrocyte spectrin.
Immunogen Type: Other
- 克隆位点
- Spe 1-2
- 亚型
- IgG1
-
- 应用备注
-
Application Note: Anti-Spectrin is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~246-280 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:40,000
Western Blot Dilution: 2-4 μg/mL
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Neutral PBS
- 缓冲液
-
Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
- 抗原
- Spectrin, Alpha, beta
- 别名
- Spectrin (alpha and beta)
- 背景
-
Synonyms: EL2, HPP, HS3, SPH3, SPTA, EL3, HS2, SPH2, HSPTB1, Erythroid alpha-spectrin
Background: Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5. This antibody is suitable for researchers interested in cytoskeleton and transcriptoion factor research.
Gene Name: SPTA1, SPTB
- 基因ID
- 6708
- NCBI登录号
- NP_003117
- UniProt
- P02549
-
