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Slc6a20a Protein (AA 1-592) (Strep Tag)

This Recombinant Slc6a20a protein is expressed in Cell-free protein synthesis (CFPS).
产品编号 ABIN3126995
发货至: 中国
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北京 101111
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Slc6a20a Protein (AA 1-592) (Strep Tag) (ABIN3126995)

抗原

Slc6a20a (SLC6A20A) (Solute Carrier Family 6 (Neurotransmitter Transporter), Member 20A (SLC6A20A))

蛋白类型

Recombinant

宿主

小鼠

资源

Cell-free protein synthesis (CFPS)

应用范围

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

纯度

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

质量等级

custom-made
  • 产品特性

    AA 1-592

    标记

    This Slc6a20a protein is labelled with Strep Tag.

    序列

    MEKARPQWGH PLQFVFACIS YAVGLGNVWR FPYLCQMYGG GSFLVPYIIM LIVEGMPLLY LELAVGQRMR QGSIGAWRTI SPYLSGVGVA SVVVSFFLSM YYNVINAWGF WYLFHSFQDP LPWSVCPLNS NHTGYDEECE KASSTQYFWY RKTLNISPSI QENGGVQWEP ALCLTLAWLM VYLCILRGTE STGKVVYFTA SMPYCVLIIY LVRGLTLHGA TNGLMYMFTP KMEQLANPKA WINAATQIFF SLGLGFGSLI AFASYNEPSN NCQKHAIIVS IINSSTSIFA SIVTFSIYGF KATFNYENCL NKVILLLTNS FDLEDGFLTV SNLEEVKNYL ASTYPNKYSE VFPHIRNCSL ESELDTAVQG TGLAFIVYTE AIKNMEVSQL WSVLYFFMLL MLGIGSMLGN TAAILTPLTD SKVISSYLPK EAISGLVCLI NCAVGMVFTM EAGNYWFDIF NDYAATLSLL LIVLVETIAV CYVYGLKRFE SDLRAMTGRT LSWYWKVMWA FVSPLLIVGL FIFYLSDYIL TGTLQYQAWD ATQGQLVTKD YPPHALAVIG LLVASSTMCI PLVALGTFIR NRLKRGGSAP VA
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    产品特性

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • 应用备注

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -80 °C

    储存方法

    Store at -80°C.

    有效期

    12 months
  • 抗原

    Slc6a20a (SLC6A20A) (Solute Carrier Family 6 (Neurotransmitter Transporter), Member 20A (SLC6A20A))

    别名

    Slc6a20a

    背景

    Sodium- and chloride-dependent transporter XTRP3A (IMINO-B) (Solute carrier family 6 member 20A) (X transporter protein 3 similar 1),FUNCTION: Mediates the Na(+)- and Cl(-)-dependent uptake of imino acids such as L-proline, N-methyl-L-proline and pipecolate as well as N-methylated amino acids (PubMed:15689184). Also transports glycine, regulates proline and glycine homeostasis in the brain playing a role in the modulation of NMDAR currents (PubMed:33428810). {ECO:0000269|PubMed:15689184, ECO:0000269|PubMed:33428810}.

    分子量

    66.2 kDa

    UniProt

    Q8VDB9
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