山羊 anti-人 Ig (Chain kappa), (Light Chain) Antibody (HRP)
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北京 101111
Quick Overview for 山羊 anti-人 Ig (Chain kappa), (Light Chain) Antibody (HRP) (ABIN969908)
抗原
标记
应用范围
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抗原表位
- Chain kappa, Light Chain
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适用
- 人
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宿主
- 山羊
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特异性
- Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins
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交叉反应 （详细）
- Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins
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产品特性
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Concentration: 1.0 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Horseradish Peroxidase -
纯化方法
- Affinity purified using solid phase human Kappa (κ) Chain
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纯度
- > 95 % based on SDS-PAGE
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免疫原
- Purified human kappa (kappa) light chain
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Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Human Ig antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human Ig antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human Ig antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human Ig antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human Ig (Chain lambda) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human Ig (Chain lambda) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human Ig (Chain kappa) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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应用备注
- This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
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说明
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HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
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浓度
- 1.0 mg/mL
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缓冲液
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
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储存液
- Kathon CG
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储存条件
- 4 °C
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储存方法
- Store freeze-dried powder at 2-8 °C. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
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- Ig
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别名
- Immunoglobulin Ig
抗原
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