山羊 anti-人 Ig (Chain kappa), (Light Chain) Antibody (Alkaline Phosphatase (AP))
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北京 101111
Quick Overview for 山羊 anti-人 Ig (Chain kappa), (Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN969902)
抗原
标记
应用范围
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抗原表位
- Chain kappa, Light Chain
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适用
- 人
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宿主
- 山羊
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特异性
- Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins
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交叉反应 （详细）
- Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins
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产品特性
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Concentration: 1.50 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Alkaline Phosphatase -
纯化方法
- Affinity purified using solid phase human Kappa (κ) Chain
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纯度
- > 95 % based on SDS-PAGE
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免疫原
- Purified human kappa (kappa) light chain
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Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Human Ig antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human Ig antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human Ig antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human Ig antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human Ig (Chain lambda) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human Ig (Chain lambda) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human Ig (Chain kappa) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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应用备注
- This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
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说明
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ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.50 mg/mL
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缓冲液
- 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Prepare working dilution prior to use and then discard.
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储存条件
- 4 °C
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储存方法
- Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
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- Ig
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别名
- Immunoglobulin Ig
抗原
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