(1 reference)Quick Overview for 小鼠 anti-人 IgG Antibody (ABIN969481)
抗原
克隆类型
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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原理
- human IgG Antibody
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产品特性
- Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
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纯化方法
- Purified antibody
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免疫原
- Human IgG was isolated from human sera and purified by chromatography.
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Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgG (Fc Region) antibody (SPRD)ELISA, FACS Monoclonal H2 IgG2b SPRD
Mouse anti-Human IgG (Fc Region) antibody (Cy5)ELISA, FACS Monoclonal H2 IgG2b Cy5
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应用备注
- ELISA: 1/10000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified antibody in PBS containing 0.03 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).
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: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).
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- IgG
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物质类
- Antibody
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背景
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Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma
cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor. -
分子量
- 50 kDa
抗原
-
