山羊 anti-人 IgG (Heavy & Light Chain) Antibody (HRP)
Quick Overview for 山羊 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN965347)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 山羊
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Supplier Product No.
- 809-1302
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Supplier
- Rockland
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原理
- Fab Human IgG (H&L) Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
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产品特性
- Fab Anti-Human IgG (H&L) Fluorescein Antibody generated in goat detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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免疫原
- Optional[Immunogen]: Human IgG whole molecule
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Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgG (Fc Region) antibody (SPRD)ELISA, FACS Monoclonal H2 IgG2b SPRD
Mouse anti-Human IgG (Fc Region) antibody (Cy5)ELISA, FACS Monoclonal H2 IgG2b Cy5
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应用备注
- Application Note: Fab Anti-Human IgG (H&L) Peroxidase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 50,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Fab Anti-Human IgG (H&L) Peroxidase Antibody generated in goat detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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