兔 anti-豚鼠 IgG (Heavy & Light Chain) Antibody (Biotin)
ELISA, IHC, WB
Polyclonal
IgG
Biotin
Fab fragment
1 image
Quick Overview for 兔 anti-豚鼠 IgG (Heavy & Light Chain) Antibody (Biotin) (ABIN965331)
抗原
IgG
片段
Fab fragment
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 豚鼠
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宿主
- 兔
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Supplier Product No.
- 806-4602
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Supplier
- Rockland
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原理
- Fab Guinea Pig IgG (H&L) Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
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产品特性
- Fab Anti-Guinea Pig IgG Peroxidase Antibody generated in rabbit detects guinea pig IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
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免疫原
- Optional[Immunogen]: Guinea Pig IgG whole molecule
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Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 0.5 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Fab Anti-Guinea Pig IgG Biotin Antibody generated in rabbit detects guinea pig IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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