兔 anti-小鸡 IgG (Heavy & Light Chain) Antibody (FITC)
Quick Overview for 兔 anti-小鸡 IgG (Heavy & Light Chain) Antibody (FITC) (ABIN965308)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 兔
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Supplier Product No.
- 803-4202
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Supplier
- Rockland
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原理
- Fab Chicken IgG (H&L) Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
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产品特性
- Fab Anti-Chicken IgG Antibody generated in rabbit detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
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免疫原
- Optional[Immunogen]: Chicken IgG whole molecule
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Goat anti-Chicken IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Chicken IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Chicken IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Chicken IgG antibody (DyLight 405)WB, FLISA, FM Polyclonal IgG DyLight 405
Goat anti-Chicken IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
Goat anti-Chicken IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Chicken IgG antibody (DyLight 405) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 405
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Fab Anti-Chicken IgG Fluorescein Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 0.5 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Fab Anti-Chicken IgG Fluorescein Antibody generated in rabbit detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
抗原
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