驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
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北京 101111
Quick Overview for 驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN965290)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 驴
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Supplier Product No.
- 713-703-168
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Supplier
- Rockland
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原理
- F(ab')2 Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
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产品特性
- F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in donkey detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to Fluorescein.
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纯化方法
- Preadsorption: Pre-Adsorbed
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免疫原
- Optional[Immunogen]: Sheep IgG whole molecule
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Sheep IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG Biotin
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应用备注
- Application Note: F(ab')2 Anti-Sheep IgG Peroxidase Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. Immunohistochemistry Dilution: 1:500 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:8,000 - 1:40,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in donkey detects immunoglobulin g from sheep, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to Peroxidase.
抗原
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