驴 anti-大鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
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北京 101111
Quick Overview for 驴 anti-大鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN965276)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 驴
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Supplier Product No.
- 712-702-149
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Supplier
- Rockland
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原理
- F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
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产品特性
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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免疫原
- Optional[Immunogen]: Rat IgG whole molecule
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Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Goat anti-Rat IgG (Heavy & Light Chain) antibody (beta-Gal) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG beta-Gal
Goat anti-Rat IgG (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Rat IgG (Heavy Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Rat IgG (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
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应用备注
- Application Note: F(ab')2 Anti-Rat IgG Fluorescein Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:500-1:2,500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 1.0 mg/mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- F(ab')2 Anti-Rat IgG (H&L) Antibody generated in donkey detects rat IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to Fluorescein.
抗原
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