山羊 anti-大鼠 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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北京 101111
Quick Overview for 山羊 anti-大鼠 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN965253)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Fc Region
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适用
- 大鼠
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宿主
- 山羊
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Supplier Product No.
- 712-1522
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Supplier
- Rockland
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原理
- F(ab')2 Rat IgG Fc Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(ab) or Bovine, Horse, and Human Serum Proteins.
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产品特性
- Anti-Rat IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically rat IgG (H&L). This secondary peroxidase conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- F(ab')2 Anti-Rat IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Rat IgG F(c) fragment
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Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 488
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Rat IgG antibody (DyLight 488)WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Goat anti-Rat IgG (Heavy Chain) antibody (beta-Gal)ELISA, IHC, WB Polyclonal IgG beta-Gal
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应用备注
- Application Note: F(ab')2 Anti-Rat IgG Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 μg of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for F(ab')2 Anti-Rat IgG Antibody. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- F(ab')2 Anti-Rat IgG F(c) Alkaline Phosphatase Antibody was generated in goat and detects specifically Rat IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Anti-Rat IgG Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
抗原
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