山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
Quick Overview for 山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN965249)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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Supplier Product No.
- 712-1233
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Supplier
- Rockland
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纯化方法
- Preadsorption: Solid phase absorption
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免疫原
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Immunogen: Rat IgG whole molecule
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Labeling Ratio
- 5.3
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Goat anti-Rat IgG antibody (DyLight 488)
WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Goat anti-Rat IgG (Heavy & Light Chain) antibody (beta-Gal) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG beta-Gal
Goat anti-Rat IgG (Heavy & Light Chain) antibody (SPRD) - PreadsorbedELISA, FACS Polyclonal IgG SPRD F(ab')2 fragment
Goat anti-Rat IgG (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Rat IgG (Heavy Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Rat IgG (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
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应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
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说明
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Excitation/Emission wavelength: 494 nm/514 nm
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 0.5 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Product is photosensitive and should be protected from light.
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储存条件
- RT,4 °C,-20 °C
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
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Synonyms: Goat F(ab')2 Anti-Rat IgG Fluorescein Conjugated Antibody, Goat F(ab')2 Anti-Rat IgG Antibody FITC Conjugation
Background: F(ab')2 Anti-Rat IgG (H&L) Antibody generated in goat detects rat IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to fluorescein.
抗原
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