电话:: +86 (0512) 65829739
传真:: +86 (010) 6788 5057
电子邮件:: orders@antibodies-online.cn

我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

驴 anti-小鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC F(ab')2 fragment

HSPB8 silencing and its effect on expression of ERα and ERβin MCF-7 cell line. (驴 anti-小鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed)

1 image

(1 reference)

产品编号 ABIN965200

Supplier Product No.: 710-702-124

发货至: 中国

Datasheet as PDF

Quick Overview for 驴 anti-小鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN965200)

抗原

IgG

片段

F(ab')2 fragment

克隆类型

多克隆

标记

FITC

应用范围

Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

亚型

IgG

抗原表位
2396

517

417

146

130

128

66

29

28

28

21

9

5

5

2

2

2

2

2

1

1

1
Heavy & Light Chain
适用
752

702

607

468

410

244

213

176

146

141

124

110

107

101

82

62

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
小鼠
宿主
1773

1583

566

282

122

112

41

32

22

21

15

11

11
驴

Supplier Product No.

710-702-124

Supplier

Rockland

原理

F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

交叉反应（详细）

Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

产品特性

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

纯化方法

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

免疫原

Optional[Immunogen]: Mouse IgG whole molecule
Goat anti-Mouse IgG (Whole Molecule) antibody (FITC)
FACS, ICC, IHC (fro), IHC (p) Polyclonal IgG FITC

Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800

Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG DyLight 488

Goat anti-Mouse IgG antibody (DyLight 800) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG DyLight 800

Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - Preadsorbed
FACS, FLISA, FM, DB Polyclonal IgG TRITC

Goat anti-Mouse IgG antibody (DyLight 680)
WB, FLISA, FM, DB Polyclonal IgG DyLight 680

Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)
IHC, FACS Polyclonal IgG Cy5

Goat anti-Mouse IgG (Heavy & Light Chain) antibody (APC) - Preadsorbed
ELISA, FACS Polyclonal IgG APC

Rabbit anti-Mouse IgG antibody (Atto 550)
WB, FLISA, FM Polyclonal IgG Atto 550

应用备注

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Piccolella, Crippa, Cristofani, Rusmini, Galbiati, Cicardi, Meroni, Ferri, Morelli, Carra, Messi, Poletti: "The small heat shock protein B8 (HSPB8) modulates proliferation and migration of breast cancer cells." in: Oncotarget, Vol. 8, Issue 6, pp. 10400-10415, (2018) (PubMed).
抗原

IgG

物质类

Antibody

背景

Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

You are here: