山羊 anti-小鼠 IgM Antibody (Alkaline Phosphatase (AP))
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Quick Overview for 山羊 anti-小鼠 IgM Antibody (Alkaline Phosphatase (AP)) (ABIN965173)
抗原
片段
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 710-1507
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Supplier
- Rockland
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原理
- F(ab')2 Mouse IgM (mu chain) Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other mouse heavy or light chain proteins.
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产品特性
- F(ab')2 Anti-Mouse IgG (H&L) Alkaline Phosphatase Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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纯化方法
- F(ab')2 Anti-Mouse IgM (mu chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Mouse IgM whole molecule
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Goat anti-Mouse IgM (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgM antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgM antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Rat anti-Mouse IgM (Chain mu) antibody (HRP)ELISA, IHC Monoclonal SB73a IgG1 HRP
Goat anti-Mouse IgM (Heavy Chain) antibody (Cy5)FACS, IHC Polyclonal IgG Cy5
Rat anti-Mouse IgM (Chain mu) antibody (Alkaline Phosphatase (AP))ELISA, IHC Monoclonal SB73a IgG1 Alkaline Phosphatase (AP)
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应用备注
- Application Note: F(ab')2 Anti-Mouse IgM (mu chain) Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 μg of Mouse IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for F(ab')2 Anti-Mouse IgM (mu chain) Antibody. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:5,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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