山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
(2 references)
Quick Overview for 山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN965171)
抗原
片段
克隆类型
标记
应用范围
亚型
-
-
抗原表位
- Heavy & Light Chain
-
适用
- 小鼠
-
宿主
- 山羊
-
Supplier Product No.
- 710-1332
-
Supplier
- Rockland
-
原理
- F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
-
交叉反应 （详细）
- Minimal crossreactivity against Bv Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
-
产品特性
- F(ab')2 Anti-Mouse IgG F(ab')2 Peroxidase Antibody generated in goat detects Mouse F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-
纯化方法
- Preadsorption: Pre-Adsorbed
-
免疫原
-
Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Immunogen Type: Native Protein
-
-
Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
-
-
应用备注
- Application Note: F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:300:000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
-
: "Cancer-related transcription regulator protein NAC1 forms a protein complex with CARM1 for ovarian cancer progression." in: Oncotarget, Vol. 9, Issue 47, pp. 28408-28420, (2018) (PubMed).
: "Precise epitope determination of the anti-vimentin monoclonal antibody V9." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 3917-3921, (2018) (PubMed).
-
: "Cancer-related transcription regulator protein NAC1 forms a protein complex with CARM1 for ovarian cancer progression." in: Oncotarget, Vol. 9, Issue 47, pp. 28408-28420, (2018) (PubMed).
-
- IgG
-
物质类
- Antibody
-
背景
- F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
-
