驴 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 709-703-123
- Supplier
- Rockland
- 原理
- F(ab')2 Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
- 产品特性
- F(ab')2 Anti-Human IgG (H&L) Fluorescein Antibody generated in donkey detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
- 纯化方法
- Preadsorption: Pre-Adsorbed
- 免疫原
- Optional[Immunogen]: Human IgG whole molecule
- 亚型
- IgG
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Fc Region) antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
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- 应用备注
- Application Note: F(ab')2 Anti-Human IgG (H&L) Peroxidase Antibody secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. Immunohistochemistry Dilution: 1:500 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- F(ab')2 Anti-Human IgG (H&L) Peroxidase Antibody generated in donkey detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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