兔 anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (PE)
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- Golden Syrian Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
- 交叉反应
- Golden Syrian Hamster
- 产品特性
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Anti-Golden Syrian Hamster IgG (H&L) Antibody generated in rabbit detects specifically golden syrian hamster IgG. This secondary Anti-Golden Syriam Hamster IgG antibody is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis. Anti-Golden Syrian Hamster IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.
Anti-Golden Syrian Hamster IgG (H&L) phycoerythrin conjugated antibody generated in rabbit detects specifically golden syrian hamster IgG. This secondary phycoerythrin conjugated antibody anti-Golden Syriam Hamster is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. - 纯化方法
- F(ab')2 Anti-Golden Syrian Hamster IgG Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
- 免疫原
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Immunogen: Anti-Golden Syrian Hamster IgG (H&L) was produced by repeated immunization with Golden Syrian Hamster IgG whole molecule in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG Texas Red (TR) F(ab')2 fragment
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFLISA, FM Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFM Polyclonal IgG Texas Red (TR)
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- 应用备注
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Application Note: Golden Syrian Hamster IgG (H&L) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Phycoerythrin Conjugation, Rabbit F(ab')2 Anti-Hamster IgG Phycoerythrin Conjugated Antibody, Rabbit Fab2 Anti-Hamster IgG PE Conjugated Antibody
Background: F(ab')2 Anti-Golden Syrian Hamster IgG Phycoerythrin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Anti-Golden Syrian Hamster IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.
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