兔 anti-小鸡 IgG (Heavy & Light Chain) Antibody (PE)
-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
-
抗原表位
- Heavy & Light Chain
-
适用
- 小鸡
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- PE
-
应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- Supplier Product No.
- 703-408-002
- Supplier
- Rockland
- 原理
- F(ab')2 Chicken IgG (H&L) Antibody Phycoerythrin conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
- 产品特性
- F(ab')2 Anti-Chicken IgG Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.
- 免疫原
- Optional[Immunogen]: Chicken IgG whole molecule
- 亚型
- IgG
-
Goat anti-Chicken IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Chicken IgG antibody (DyLight 405)WB, FLISA, FM Polyclonal IgG DyLight 405
Goat anti-Chicken IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Chicken IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Chicken IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
Goat anti-Chicken IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Chicken IgG antibody (DyLight 405) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 405
Donkey anti-Chicken IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Donkey anti-Chicken IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedWB, FLISA, FM Polyclonal IgG TRITC
-
- 应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 0.5 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-
