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山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 532) - Preadsorbed

Name: 山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 532) - Preadsorbed
SKU: ABIN965001
Availability: OutOfStock

WB, FLISA, FM, DB Polyclonal IgG Atto 532

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) . (山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 532) - Preadsorbed)

Immunofluorescence (IF) image for Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 532) - Preadsorbed (ABIN965001) (山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (Atto 532) - Preadsorbed)

2 images

产品编号 ABIN965001

Supplier Product No.: 612-153-120s

发货至: 中国

Datasheet as PDF

抗原 See all IgG products

IgG
抗原表位
2397

515

417

146

129

128

67

29

28

28

21

9

5

5

2

1

1
Heavy & Light Chain
适用
687

677

573

432

381

208

195

144

127

126

107

102

96

88

82

58

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1
大鼠
宿主
1705

1431

525

205

112

111

32

31

21

18

15

11

11
山羊
克隆类型
3130

172

16

1
多克隆
标记
587

416

415

367

274

262

235

118

85

72

67

64

61

58

58

44

39

39

37

35

35

32

31

31

30

28

28

28

28

28

28

28

27

27

26

26

25

23

21

21

19

17

17

16

15

15

15

15

13

10

8
Atto 532
应用范围
2039

1985

1823

1778

998

919

895

487

423

149

98

64

56

51

45

30

28

25

20

15

11

9

8

8

8

7

5

5

4

4

4

3

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.

612-153-120s

Supplier

Rockland

原理

Rat IgG (H&L) Antibody ATTO 532 Conjugated Pre-Adsorbed

交叉反应（详细）

Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.

产品特性

Anti-Rat IgG (H&L) generated in goat detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to alkaline phosphatase.

纯化方法

Rat IgG (H&L) Antibody ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Optional[Immunogen]: Rat IgG whole molecule

亚型

IgG
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Rabbit anti-Mouse IgG antibody (HRP) - Preadsorbed
ELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment

Goat anti-Rat IgG antibody (DyLight 488)
WB, FLISA, FM Polyclonal IgG DyLight 488

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 647N

Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG antibody (Cy3) - Preadsorbed
WB, FACS, FLISA, FM, DB Polyclonal IgG Cy3

Goat anti-Rat IgG (Heavy Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated

Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

应用备注

Application Note: Anti-Rat IgG (H&L) conjugated to ATTO 532 has been tested by dot blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

IgG

Abstract

IgG 产品

物质类

Antibody

背景

Anti-Rat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.