兔 anti-Pig IgG (Heavy & Light Chain) Antibody (FITC)
WB, FACS, FLISA, FM
Polyclonal
IgG
FITC
F(ab')2 fragment
1 image
产品编号 ABIN964877
Supplier Product No.: 314-4202
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-Pig IgG (Heavy & Light Chain) Antibody (FITC) (ABIN964877)
抗原
IgG
片段
F(ab')2 fragment
克隆类型
多克隆
标记
FITC
应用范围
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- Pig
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宿主
- 兔
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Supplier Product No.
- 314-4202
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Supplier
- Rockland
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原理
- F(ab')2 Swine IgG (H&L) Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Swine IgG and Swine Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
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产品特性
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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纯化方法
- This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
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免疫原
- Optional[Immunogen]: Swine IgG whole molecule
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Goat anti-Pig IgG (Heavy & Light Chain) antibody (FITC)
WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
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应用备注
- Application Note: F(ab')2 Anti-Swine IgG Antibody Fluorescein Conjugate has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Thimerosal -
储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
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