山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (HRP)
-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
-
抗原表位
- Heavy & Light Chain
-
适用
- 小鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- HRP
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 310-1302
- Supplier
- Rockland
- 原理
- F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
- 产品特性
- F(ab')2 Anti-Mouse IgG (H&L) Fluorescein Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
- 纯化方法
- This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
- 免疫原
- Optional[Immunogen]: Mouse IgG whole molecule
- 亚型
- IgG
-
Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
-
- 应用备注
- Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
- 浓度
- 10.0 mg/mL
- 缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-
