兔 anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (FITC)
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Quick Overview for 兔 anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (FITC) (ABIN964836)
抗原
片段
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- Golden Syrian Hamster
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宿主
- 兔
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Supplier Product No.
- 307-4202
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Supplier
- Rockland
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原理
- F(ab')2 Golden Syrian Hamster IgG (H&L) Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
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产品特性
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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纯化方法
- This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
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免疫原
- Optional[Immunogen]: Hamster IgG whole molecule
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Goat anti-Armenian Hamster IgG antibody (DyLight 800) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 800
Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) antibody (Texas Red (TR))WB, FACS, FLISA, FM Polyclonal IgG Texas Red (TR) F(ab')2 fragment
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedWB, FLISA, FM Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFM, DB Polyclonal IgG Texas Red (TR)
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应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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