兔 anti-山羊 IgG (Heavy & Light Chain) Antibody (HRP)
Quick Overview for 兔 anti-山羊 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN964827)
抗原
片段
克隆类型
标记
应用范围
亚型
-
-
抗原表位
- Heavy & Light Chain
-
适用
- 山羊
-
宿主
- 兔
-
Supplier Product No.
- 305-4302
-
Supplier
- Rockland
-
原理
- F(ab')2 Goat IgG (H&L) Antibody Peroxidase Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
-
产品特性
- F(ab')2 Anti-Goat IgG Fluorescein Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
-
纯化方法
- This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
-
免疫原
- Optional[Immunogen]: Goat IgG whole molecule
-
-
Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Pig anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Guinea Pig anti-Goat IgG antibodyELISA, WB Polyclonal IgG unconjugated
Chicken anti-Goat IgG antibody (DyLight 488)WB, IF Polyclonal IgY DyLight 488
Pig anti-Goat IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA Polyclonal IgG PE
-
-
应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
-
浓度
- 10.0 mg/mL
-
缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- IgG
-
物质类
- Antibody
-
背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
-
