兔 anti-犬 IgG (Heavy & Light Chain) Antibody (HRP)
ELISA, IHC, WB
Polyclonal
IgG
HRP
F(ab')2 fragment
产品编号 ABIN964822
Supplier Product No.: 304-4302
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-犬 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN964822)
抗原
IgG
片段
F(ab')2 fragment
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
-
-
抗原表位
- Heavy & Light Chain
-
适用
- 犬
-
宿主
- 兔
-
Supplier Product No.
- 304-4302
-
Supplier
- Rockland
-
原理
- F(ab')2 Dog IgG (H&L) Antibody Peroxidase Conjugated
-
交叉反应 （详细）
- The F(ab')2 fragment of a rabbit IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
-
产品特性
- F(ab')2 Anti-Dog IgG F(ab')2 Fluorescein Antibody generated in rabbit detects Dog F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-
纯化方法
- F(ab')2 Anti-DOG IgG Antibody is Peroxidase Conjugated.
-
免疫原
- Optional[Immunogen]: Dog IgG whole molecule
-
-
Mouse anti-Dog IgG antibody
Animal-Free ELISA Monoclonal AB169-1-E12 IgG2a Recombinant Antibody unconjugated
-
-
应用备注
- Application Note: Dog antibody has been assayed against 1.0 μg of Dog IgG in a standard capture ELISA. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for anti-dog antibody. F(ab')2 Anti-Dog IgG is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
-
浓度
- 10.0 mg/mL
-
缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:None -
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- IgG
-
物质类
- Antibody
-
背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F(ab')2 Anti-Dog antibody is ideal for investigators involved in serum protein component research.
抗原
-
You are here:
