兔 anti-Cat IgG (F(ab')2 Region) Antibody
ELISA, IHC, WB
Polyclonal
IgG
unconjugated
F(ab')2 fragment
Quick Overview for 兔 anti-Cat IgG (F(ab')2 Region) Antibody (ABIN964809)
抗原
IgG
片段
F(ab')2 fragment
克隆类型
多克隆
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- F(ab')2 Region
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适用
- Cat
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宿主
- 兔
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Supplier Product No.
- 302-4104
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Supplier
- Rockland
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原理
- F(ab')2 Cat IgG F(ab')2 Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgG, Cat IgG F(ab')2 and Cat Serum. No reaction was observed against Cat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
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产品特性
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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纯化方法
- This product is a F(ab')2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.
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免疫原
- Optional[Immunogen]: Cat IgG F(ab')2 fragment
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Goat anti-Cat IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Mouse anti-Cat IgG antibodyAnimal-Free ELISA Monoclonal AB171-2-B03 IgG2a Recombinant Antibody unconjugated
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应用备注
- Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Immunohistochemistry Dilution: 1:1,000-1:5,000 Western Blot Dilution: 1:2,000-1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- F(ab')2 Anti-Cat IgG F(ab')2 Antibody generated in rabbit detects Cat F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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