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小鼠 anti-人 IgM Antibody (APC)

FACS Monoclonal CH2 IgG1 kappa APC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgM (CH2) APC antibody (concentration in sample 0,6 μg/mL). (小鼠 anti-人 IgM Antibody (APC))

Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. (小鼠 anti-人 IgM Antibody (APC))

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human IgM (CH2) APC antibody (concentration in sample 0,6 μg/mL) and anti-human CD19 (LT19) PE antibody (20 μL reagent / 100 μL of peripheral whole blood). (小鼠 anti-人 IgM Antibody (APC))

Separation of human IgM positive B cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using using anti-human IgM (CH2) APC antibody (concentration in sample 0,6 μg/mL). (小鼠 anti-人 IgM Antibody (APC))

4 images

产品编号 ABIN94404

发货至: 中国

Datasheet as PDF

Quick Overview for 小鼠 anti-人 IgM Antibody (APC) (ABIN94404)

抗原

IgM

克隆类型

单克隆

标记

APC

应用范围

391
295
295
256
183
145
91
91
64
62
54
53
47
38
18
17
10
6
5
3
3
3
2
2
2
1
1
1
1
1

Flow Cytometry (FACS)

克隆位点

CH2

亚型

IgG1 kappa

适用
231

167

92

42

34

34

30

24

17

14

13

13

13

13

12

4

2

2
人
宿主
373

241

90

22

14

5

4

1

1
小鼠

原理

Anti-Hu IgM APC

特异性

The antibody CH2 reacts with Fc fragment of human IgM.

交叉反应（详细）

Human

纯化方法

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Purified human IgM.
Mouse anti-Human IgM antibody (FITC)
FACS Monoclonal CH2 IgG1 kappa FITC

Goat anti-Human IgM antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Mouse anti-Human IgM antibody (PE)
FACS Monoclonal CH2 IgG1 kappa PE

Mouse anti-Human IgM antibody (iFluor™647)
GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™647

Mouse anti-Human IgM antibody (iFluor™700)
GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™700

Mouse anti-Human IgM antibody (mFluor™450)
GMP Grade FACS Monoclonal M-2E6 IgG1 mFluor™450

Mouse anti-Human IgM antibody (SPRD)
ELISA, FACS Monoclonal UHB IgG3 SPRD

Mouse anti-Human IgM antibody (PE-Cy7)
GMP Grade FACS Monoclonal M-2E6 IgG1 PE-Cy7

Goat anti-Human IgM antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Human IgM antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

应用备注

Flow cytometry: Recommended dilution: 1-4 μg/mL. Extracellular and intracellular staining.

限制

仅限研究用
状态

Liquid

浓度

0.1 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
抗原

IgM

物质类

Antibody

背景

Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.,immunoglobulin M

分子量

900 kDa

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