山羊 anti-猴 IgA (Free & Bound), (Secretory Component) Antibody (Biotin)
WB, IF, IHC (fro), IP, EIA, ID, DB
Polyclonal
IgG
Biotin
Quick Overview for 山羊 anti-猴 IgA (Free & Bound), (Secretory Component) Antibody (Biotin) (ABIN784368)
抗原
IgA
克隆类型
多克隆
标记
Biotin
应用范围
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB)
亚型
IgG
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抗原表位
- Free & Bound, Secretory Component
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适用
- 猴
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宿主
- 山羊
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产品特性
- Molar Ratio: Biotin / IgG aprox. 8.6
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纯化方法
- DEAE-column Chromatography
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免疫原
- Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 1 mL sterile distilled water
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浓度
- 10.0 mg/mL
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缓冲液
- PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- IgA
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物质类
- Antibody
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背景
- Secretory component is present in Monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimmer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.Synonyms: Monkey Immunoglobulin A
抗原
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