山羊 anti-豚鼠 IgG2 Antibody (HRP)
WB, IF, EIA, IHC (fro), IP, ID, DB
Polyclonal
IgG
HRP
Quick Overview for 山羊 anti-豚鼠 IgG2 Antibody (HRP) (ABIN783017)
抗原
IgG2
克隆类型
多克隆
标记
HRP
应用范围
Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunodiffusion (ID), Dot Blot (DB)
亚型
IgG
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适用
- 豚鼠
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宿主
- 山羊
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产品特性
- Molar Ratio: Peroxidase/IgG aprox. 1.7
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纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography. It is free of other serum proteins as tested by immunoelectrophoresis
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免疫原
- Purified IgG2 isolated from Guinea Pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore by adding 1.0 mL of sterile distilled water
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浓度
- 8.3 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
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储存液
- Without preservative
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注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
储存条件
- 4 °C/-20 °C
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储存方法
- Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -28 °C for longer.
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- IgG2
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物质类
- Antibody
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背景
- Synonyms: Guinea Pig Immunoglobulin G2
抗原
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