绵羊 anti-人 IgA (Free & Bound), (Secretory Component) Antibody (HRP)
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北京 101111
Quick Overview for 绵羊 anti-人 IgA (Free & Bound), (Secretory Component) Antibody (HRP) (ABIN7891014)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Free & Bound, Secretory Component
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适用
- 人
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宿主
- 绵羊
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特异性
- Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In immunocytochemical and immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in human body fluids.
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免疫原
- Secretory component can be present in Human secretions bound to secretory IgA (sIgA) and in free form. Free Human secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Mouse anti-Human IgA (Heavy Chain) antibody
ELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Rabbit anti-Human IgA (Chain alpha), (Heavy Chain) antibodyELISA, IHC, WB unconjugated
Mouse anti-Human IgA antibodyELISA, IHC (p) Monoclonal AD2-947 IgG1 unconjugated
Goat anti-Human IgA antibody (HRP)ELISA, WB, ICC, IHC (fro), IHC (p), ELISpot Polyclonal IgG HRP
Goat anti-Human IgA antibody (Biotin)ELISA, WB, FACS, ICC, IHC (fro), IP, IHC (p), ELISpot, MA Polyclonal IgG Biotin
Goat anti-Human IgA antibody (FITC)WB, FACS, ICC, IHC (fro), IHC (p), FLISA Polyclonal IgG FITC
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应用备注
- This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The antibody is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
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缓冲液
- Horseradish peroxidase-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized conjugate can be stored at +4°C short term; prolonged storage at or below -20°C.
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- IgA
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物质类
- Antibody
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背景
- Synonyms: Human Immunoglobulin A
抗原
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