小鼠 anti-人 IgM (Heavy Chain) Antibody
IHC (p)
Monoclonal
SPM188
IgG1
unconjugated
产品编号 ABIN7879459
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 小鼠 anti-人 IgM (Heavy Chain) Antibody (ABIN7879459)
抗原
IgM
克隆类型
单克隆
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
SPM188
亚型
IgG1, kappa
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抗原表位
- Heavy Chain
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适用
- 人
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宿主
- 小鼠
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原理
- Anti-IgM Antibody
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纯化方法
- Protein G affinity chromatography
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免疫原
- Heavy chain of human IgM was used as the immunogen for the Anti-IgM antibody.
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应用备注
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Optimal dilution of the Anti-IgM antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Anti-IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- IgM
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物质类
- Antibody
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背景
- Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
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UniProt
- P01871, P20769
抗原
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