山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Poly-HRP)
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北京 101111
Quick Overview for 山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (Poly-HRP) (ABIN7849474)
抗原
克隆类型
标记
应用范围
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠, 兔
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宿主
- 山羊
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原理
- Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) HRP polymer labeled
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特异性
- By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.
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纯化方法
- Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
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免疫原
- Mouse/Rabbit IgG
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
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应用备注
- ELISA: 1/5000 - 1/20000, WB: 1/5000 - 1/20000, IH: 1/100 - 1/500, IC: 1/100 - 1/500
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 5 mg/mL
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缓冲液
- Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1 % BSA, 50 % glycerol, 0.05 % Proclin
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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- IgG
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物质类
- Antibody
抗原
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