大鼠 anti-山羊 IgG Antibody
Our Local Distributor
北京 101111
Quick Overview for 大鼠 anti-山羊 IgG Antibody (ABIN7595373)
抗原
克隆类型
应用范围
亚型
-
-
适用
- 山羊
-
宿主
- 大鼠
-
原理
- Rat anti goat IgG. Functions as an adapter to convert a primary antibody from one species into another.
-
特异性
- Specific for goat IgG
-
纯化方法
- Purified
-
-
Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Pig anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Guinea Pig anti-Goat IgG antibodyELISA, WB Polyclonal IgG unconjugated
Chicken anti-Goat IgG antibody (DyLight 488)WB, IF Polyclonal IgY DyLight 488
-
-
实验流程
-
Western Blot Protocol
- Run the SDS-PAGE at 250V.
- Soak the gel for 15 minutes in transfer buffer, then rinse with DI water.
- Soak the membrane for 5 minutes in DI water.
- Semi-dry transfer at 10V.
- After transfer, rinse the membrane once with PBS, then wash 3× for 5 minutes each with PBS (shaking at 50 RPM).
- Block with 1× PBS containing 1% LFDM for 30 minutes at room temperature (shake at 50 RPM).
- Rinse with 70 ml of PBS.
- Add your primary antibodies and incubate overnight in the fridge (3–5°C).
- Wash the membrane 5 times (5 minutes each) with PBST containing 0.1% Tween-20 (use 100 ml, shake at 50 RPM).
- Add the Adaptor-Secondary of your choice at 1:5000 dilution and incubate with shake for 30 minutes.
- Dip in DI water (or 1x PBS).
- Incubate with your secondary antibody for example, HRP-Rb anti-Ch (diluted 1:20,000). Use 5 ml per membrane (6×8 cm). Incubate for 30 minutes to 1 hour at room temperature, shaking at 50 RPM.
- Wash 4 times (5 minutes each) with 100 ml of PBST, shaking at 50 RPM.
- Wash 6 more times with 100 ml of PBS, shaking at 50 RPM.
- Add ECL substrate.
- Scan the blot on a C-Digit at high resolution (12 minutes).
- Cut the issue at 5 µm
- Drain and Dry
- Dewax and Dehydrate
- HIER using Thermo's PT Module - HIER buffer: Tris-EDTA pH 9.00
- Stain the slides: Buffer wash - Peroxide Block (5 min) - Buffer wash (2x) - Protein Block (5 min) - Buffer wash (2x) - Primary antibody (30 min) - Buffer wash (2x) - Adaptor-Secondary - Buffer wash - HRP-Polymer (30 min) - Buffer wash (3x) - Water wash (1x)
- Add DAB
- Counterstain
- Coverslip
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute in 500 µL DI water.
-
缓冲液
- Contains Signal Enhancer and Background Quencher. Preservative: 0.02% benzalkonium chloride
-
储存液
- Benzalkonium chloride
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term.
-
有效期
- 12 months
-
-
- IgG
抗原
-
