Recombinant 小鼠 anti-兔 IgG Antibody
Quick Overview for Recombinant 小鼠 anti-兔 IgG Antibody (ABIN7581783)
抗原
抗体类型
克隆类型
应用范围
质量等级
亚型
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适用
- 兔
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宿主
- 小鼠
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Expression System
- Phage display
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原理
- recombinant Multiclonal anti-rabbit IgG
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特异性
- This is an antibody developed by antibody phage display technology using a human naive antibody gene library and rabbit IgG antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.
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无交叉反应
- Cat, 犬, 人, 兔
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交叉反应 （详细）
- No known cross reactivity. No cross reactivtiy to human IgG, rabbit IgG, dog IgG, cat IgG. Other species were not tested.
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产品特性
- Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all subclasses of rabbit IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.
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纯化方法
- Protein A purification
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免疫原
- No immunization, animal-free antibody development
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Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Mouse anti-Rabbit IgG antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
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应用备注
- Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4, 0,02 % ProClin300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- IgG
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物质类
- Antibody
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分子量
- 150 kDa
抗原
-
