Recombinant 小鼠 anti-人 IgG (Fc Region) Antibody
-
- 抗原 See all IgG products
- IgG
- 抗体类型
- Recombinant Antibody
-
抗原表位
- Fc Region
-
适用
- 人
-
宿主
- 小鼠
- Expression System
- Phage display
-
克隆类型
- Multiclonal
-
应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- Mouse anti-human IgG (Fc-specific) Antibody, animal-free multiclonal Ab
- 特异性
- This is an antibody developed by antibody phage display technology using a human naive antibody gene library and human IgG as antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.
- 无交叉反应
- Cat, Cow, 犬, 山羊, 马, 人, 小鼠, 兔, 大鼠
- 交叉反应 （详细）
- No known cross reactivity. No cross-reactivity to human IgM, human IgE, human IgA, mouse IgG, rabbit IgG, horse IgG, bovine IgG, goat IgG, rat IgG, dog IgG, cat IgG. Other species were not tested.
- 产品特性
- Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all four different subclasses of human IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.
- 纯化方法
- Protein A purification
- 质量等级
- Animal-Free
- 免疫原
- No immunization, animal-free antibody development. Antigen: human intravenous immunoglobulins (IVIg)
- 亚型
- IgG2a
-
Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG2b PE
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
-
- 应用备注
- Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH 7.4,
- 储存条件
- -20 °C
-
-
