Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins
纯化方法
Produced in: E.coli
免疫原
sdAbs anti-chicken IgY binds strongly and specifically to all IgYs. The anti-Chicken IgY sdAb is a single-domain anitbody against Chicken IgY, that can be labeled or immobilized by the customer.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C
缓冲液
sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
The immunorepertoire of chickens is much simpler compared to any other mammal, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.